Composite

Part:BBa_K804011

Designed by: Joe Ho   Group: iGEM12_British_Columbia   (2012-10-02)


Constitutive EYFP generator - Arabinose inducible MetA coding gene

This part contains a constitutive EYFP generator along with an arabinose inducible (Pbad) Met A coding gene. Upon induction with arabinose, MetA is expressed for for the biosynthesis of de novo methionine. It encodes for the MetA enzyme which transfers a succinyl group from succinyl-CoA to homoserine, forming O-succinyl-L-homoserine, a precursor of methionine. This construct can be used to induce growth in Met- auxotrophs and can be monitored by fluorescence for its population dynamics in co-culture with other auxotrophs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 994
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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